Skip to main content
Anaerobic Blood Agar Plates Compared | Oxyrase

Anaerobic Blood Agar Plates That Work for You

Active enzyme protection. Bench-top stability. Faster recovery.

2x
Recovery vs. conventional media
2 hrs
Bench-top O₂ stability
4 mo
OxyPRAS Plus shelf life
3 mo
OxyPlate shelf life

Not all anaerobic blood agar plates are created equal. Specifically, conventional plates lose their reduced state within minutes of opening. OxyPRAS Plus and OxyPlate, however, take a different approach. Both use an active enzyme embedded in the agar to scavenge oxygen in real time. As a result, your lab gets better recovery, longer shelf life, and a simpler workflow than conventional anaerobic blood agar.

Two Anaerobic Blood Agar Product Lines. One Enzyme System.

Anaerobic Blood Agar Workflow Comparison

1 Open 2 Streak 3 Incubate

OxyPlate

Simply open, streak at the bench, and incubate.

In other words, no jar, chamber, or post-reduction is needed.

Simplest Workflow | 3 Steps
1 Open 2 Streak 3 Load jar or pouch 4 Incubate

OxyPRAS Plus

Open, streak at the bench, load a jar or pouch, then incubate.

As a result, post-reduction is eliminated with 2 hrs of bench-top flexibility.

Automation Ready | 4 Steps
1 Post- reduce 2 Open 3 Streak in chamber 4 Seal in jar 5 Incubate

Standard Static PRAS / Conventional

Post-reduce, open, streak in the chamber, seal in a jar, then incubate.

Because there is no active protection, speed is critical to limit O₂ exposure.

Legacy Workflow | 5 Steps

How the Oxyrase Enzyme System Protects the Plate

OxyPRAS Plus / OxyPlate Agar with Active Oxyrase Enzyme O₂ O₂ O₂ O₂ REDUCED | Protected for 2 hrs Luxuriant colony growth Standard Static PRAS Plate Agar (no active O₂ removal) O₂ O₂ O₂ O₂ OXIDIZED | Exposed in minutes Reduced colony recovery Enzyme embedded in agar Actively scavenges O₂ as it enters No protection mechanism O₂ saturates the media freely

See the Difference

The photos below show colony shape and recovery on OxyPRAS Plus anaerobic blood agar compared to standard media. In each image, the left plate is conventional media while the right plate is OxyPRAS Plus. As you can see, enzyme-protected anaerobic blood agar consistently produces larger, more defined colonies. Furthermore, these results are repeatable across a wide range of fastidious organisms. For additional data, visit our anaerobic blood agar comparison studies.

Porphyromonas levii colony growth on anaerobic blood agar, OxyPRAS Plus vs conventional
Porphyromonas levii
Fusobacterium nucleatum recovery on anaerobic blood agar, OxyPRAS Plus vs conventional
Fusobacterium nucleatum
Bacteroides fragilis isolation on anaerobic blood agar, OxyPRAS Plus vs conventional
Bacteroides fragilis

Anaerobic Blood Agar Plates: Side-by-Side Comparison

Feature OxyPRAS Plus OxyPlate Standard Static PRAS Gas-Flush Packaged Conventional
Active O₂ removal in media Yes Yes No No No
Bench-top O₂ stability Up to 2 hrs Up to 2 hrs Minutes Minutes None
Bench-top streaking Yes Yes Chamber recommended Chamber recommended Chamber required
Anaerobic incubation method Jar, pouch, or chamber Self-contained Jar, pouch, or chamber Jar, pouch, or chamber Jar, pouch, or chamber
Post-reduction needed No No Yes Yes Yes
Automated streaker validated Yes Not yet validated Check with mfr. Check with mfr. Check with mfr.
Shelf life 4 months (2-8 °C) 3 months (2-8 °C) ~90 days Varies ~70 days
Workup packs 2-packs, 3-packs, bi-plates Individual plates Some suppliers Some suppliers Limited
Pricing Request a quote Request a quote Contact supplier Contact supplier Contact supplier
Total cost matters. When comparing anaerobic blood agar options, factor in gas-generating sachets, jars, and chamber maintenance in addition to plate price. Use the Oxyrase Cost Calculator to model your overall anaerobic blood agar savings.

How Shelf Life Compares Across Anaerobic Blood Agar Types

Longer shelf life means less waste and fewer reorders. Consequently, the anaerobic blood agar manufacturing method you choose has a direct impact on inventory costs. Below is how each type of anaerobic blood agar stacks up.

Shelf Life Comparison

OxyPRAS Plus OxyPlate Standard Static PRAS Gas-Flush Packaged Conventional 4 months 3 months ~90 days Varies ~70 days

Available Formulations

Both anaerobic blood agar product lines come in multiple formulations to fit different clinical needs. For instance, OxyPRAS Plus covers seven anaerobic blood agar media types including bi-plates and multi-packs, while OxyPlate focuses on three core formulations. Below is a quick look at what is available.

FormulationPurposeOxyPRAS PlusOxyPlate
Brucella Blood AgarPrimary nonselective isolationIndividual, 2-pack, 3-pack
PEA Blood AgarSelective for gram-positive anaerobesIndividual, 3-packIndividual
KVL Blood AgarSelective for gram-negative anaerobesIndividual, 3-packIndividual
BBE AgarB. fragilis group selectionIndividual
BBE/KVL Bi-PlateTwo media, one plateBi-plate
StrictAna Blood AgarMost O₂-sensitive anaerobesIndividual
Schaedler Blood AgarEnriched nonselective alternativeIndividual

View product inserts for complete specifications, or download our plated media sample guide.

Frequently Asked Questions

What is the difference between OxyPRAS Plus and OxyPlate?

Both product lines use the Oxyrase Enzyme System and offer 2-hour bench-top stability. However, they serve different lab needs. OxyPRAS Plus provides the widest formulation range, workup packs, automation validation, and a 4-month shelf life. In contrast, OxyPlate delivers the simplest workflow: open, streak, and incubate with no jar, chamber, or post-reduction required. It carries a 3-month shelf life. For a full organism list, see supported microorganisms.

Do these plates need an anaerobic chamber or jar?

It depends on which anaerobic blood agar product line you choose. OxyPlate is fully self-contained from inoculation through incubation, so no anaerobic equipment is needed at any step. OxyPRAS Plus, on the other hand, can be streaked at the bench without a chamber but still requires a jar, pouch, or chamber for anaerobic incubation. Either way, the 2-hour bench-top stability of our anaerobic blood agar gives your team significant processing flexibility.

How does the Oxyrase Enzyme System work?

The enzyme is embedded directly in the agar, where it actively consumes dissolved oxygen in real time. Because of this, the media stays reduced even after exposure to air. By comparison, standard static PRAS plates have no active mechanism and begin re-oxidizing immediately once opened. Learn more about the Oxyrase Enzyme System.

What is the shelf life of anaerobic blood agar plates?

Shelf life varies by manufacturing method. OxyPRAS Plus anaerobic blood agar lasts 4 months at 2-8 °C, while OxyPlate lasts 3 months under the same conditions. In comparison, standard static PRAS runs approximately 90 days, and conventional plates typically expire in 70 days or less.

Try It in Your Lab

To get started, request free anaerobic blood agar sample plates or calculate your per-test savings.

Request Free Samples Cost Calculator

Microbiology Plated Media Samples

Please fill out the form below to discover the Oxyrase difference.
Please enable JavaScript in your browser to complete this form.
Step 1 of 2
Name
Organization Type
Shipping Address

Ask a Question

How can we help you? Ask a question or request more information from us!
Please enable JavaScript in your browser to complete this form.
Name

Newsletter

Please enable JavaScript in your browser to complete this form.
Name