Product Description
OxyPRAS® BHI Broth is a pre-reduced anaerobically sterilized(PRAS), nutritionally enriched liquid medium used for the cultivation of a wide variety of microorganisms.
Anaerobic sterilization of a reduced medium prevents the formation of undesirable oxidation products that inhibit the growth of anaerobes. OxyPRAS® BHI Broth is supplemented with Vitamin K1 and Hemin to support the growth of fastidious anaerobes. OxyPRAS BHI Broth is cell-free and will not interfere with Gram stain interpretation of cultures grown in it.
Features and Benefits
- Manufactured PRAS, ensuring optimal conditions for the cultivation of fastidious anaerobes.
- Arrive ready-to-use out of the package.
- 5 mL broth volume for convenient inoculation and cultivation.
- Optimal media for the use of Oxyrase® for Broth, eliminating the need for anaerobic chambers, jars, and bags.
- No end user QC: Exempt per the CLSI M22-A3 Standard.
Storage and Handling
- Storage: Store product at 2°C to 25°C in the dark. Do not shake or agitate these tubes. Product expires 1 year after the date of manufacture.
- Instructions for use: OxyPRAS BHI Broth is ready for use, and no further preparation is necessary. However, for anaerobe samples, the addition of Oxyrase® for Broth is needed to establish and maintain an anaerobic environment.
- For liquid specimens: inoculate tube(s) with 1 drop of specimen using a sterile pipette without mixing, and recap tube(s).
- For swab specimens: insert the swab into broth after inoculation. Aseptically, squeeze the swab against the side of the tube without mixing, and remove the swab and recap the tube.
Quality Control Performed
Organism, ATCC #, Results
F. nucleatum 25586: growth/hemolysis
P. levii 29147: growth / turbid
P. anaerobius 27337: growth / turbid
B. adolescentis 15703: growth / turbid
F. naviforme 25832: growth / turbid
– wet mount – – cell free –
